Enhancing Biosynthesis and Manipulating Flux in Whole Cells with Abiotic Catalysis

Metabolic engineering uses genetic strategies to drive flux through desired pathways. Recent work with electrochemical, photochemical, and chemocatalytic setups has revealed that these systems can also expand metabolic pathways and manipulate flux in whole cells. Electrochemical systems add or remove electrons from metabolic pathways to direct flux to more- or less-reduced products. Photochemical systems act as synthetic light-harvesting complexes and yield artificial photosynthetic organisms. Biocompatible chemocatalysis increases product scope, streamlines syntheses, and yields single-flask processes to deliver products that would be challenging to synthesize through biosynthetic means alone. Here, we exclusively highlight systems that combine abiotic systems with living whole cells, taking particular note of strategies that enable the merger of these typically disparate systems.