肌发生
小RNA
生物
计算生物学
RNA干扰
核糖核酸
非编码RNA
细胞生物学
遗传学
心肌细胞
基因
作者
Jen‐Hao Yang,Dimitrios Tsitsipatis,Myriam Gorospe
摘要
Abstract Long noncoding (lnc)RNAs modulate gene expression programs in a range of developmental processes in different organs. In skeletal muscle, lncRNAs have been implicated in myogenesis, the process whereby muscle precursor cells form muscle fibers during embryonic development and regenerate muscle fibers in the adult. Here, we discuss OIP5‐AS1 , a lncRNA that is highly expressed in skeletal muscle and is capable of coordinating protein expression programs during myogenesis. Given that several myogenic functions of OIP5‐AS1 involve interactions with MEF2C mRNA and with the microRNA miR‐7, it was critical to carefully evaluate the precise levels of OIP5‐AS1 during myogenesis. We discuss the approaches used to examine lncRNA copy number using OIP5‐AS1 as an example, focusing on quantification by quantitative PCR analysis with reference to nucleic acids of known abundance, by droplet digital (dd)PCR measurement, and by microscopic visualization of individual lncRNAs in cells. We discuss considerations of RNA stoichiometry in light of developmental processes in which lncRNAs are implicated. This article is categorized under: Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs
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