CfErp3 regulates growth, conidiation, inducing ipomeamarone and the pathogenicity of Ceratocystis fimbriata

The Erp3 protein, which is an important member of the p24 family, is primarily responsible for the transport of cargo from the ER to the Golgi apparatus in Saccharomyces cerevisiae. However, the function of Erp3 in plant pathogenic fungi has not been reported. In this study, we characterized the ERP3 gene in Ceratocystis fimbriata, which causes the devastating disease sweetpotato black rot. The ΔCferp3 mutants exhibited slow growth, reduced conidia production, attenuated virulence, and reduced ability to induce host to produce toxins. Further analysis revealed that CfErp3 was localized in the ER and vesicles and regulated endocytosis, cell wall integrity, and osmotic stress responses, modulated ROS levels, and the production of ipomeamarone during pathogen-host interactions. These results indicate that CfErp3 regulates C. fimbriata growth and pathogenicity as well as the production of ipomeamarone in sweetpotato by controlling endocytosis, oxidative homeostasis, and responses to cell wall and osmotic stresses.