Statherin-derived peptide protects against intrinsic erosive enamel wear in situ

Introduction: This in situ study investigated the protective effect of a solution containing statherin-derived peptide (StatpSpS) against enamel intrinsic erosion. Methods: Fifteen volunteers wore appliances containing 2 bovine specimens. The samples were subjected to erosion (ERO) with HCl, mimicking dental erosion by intrinsic acid. The volunteers participated in 3 phases (double-blind and crossover): 1) Deionized water (negative control); 2) Commercial solution containing SnCl2/NaF/AmF (800 ppm Sn+2, 500 ppm F-, pH 4.5) - (positive control); 3) Solution containing 1.88 X 10-5M StatpSpS. Four times a day, the volunteers administered one drop of the solutions (50 µl, 1 min) on each specimen. After the treatment, erosive challenges were performed extraorally with 0.01 M HCl (pH 2.0, 4 times/day, 1 min, 150 mL). Enamel wear was assessed by profilometry. Data was analyzed by one-way RM-ANOVA/Bonferroni’s tests (p<0.05). Results: In terms of the treatments, both the commercial solution - SnCl2/NaF/AmF and StatpSpS significantly reduced the wear when compared to the negative control (p<0.01), without significant differences between them (p>0.05). Conclusion: The solution containing StatpSpS demonstrated protection against enamel intrinsic erosive wear. This study marks a significant advancement in the prevention of intrinsic erosive wear, utilizing StatpSpS in acquired pellicle engineering procedures.