Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE Cells

In order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited.This study investigated the effect of LUT-STE on antioxidant activity of H 2 O 2 -induced human retinal pigment epithelial cells (ARPE) cells.LUT and LUT-STE (final concentration of 5 μg/mL) significantly enhanced cell viability from (74.84 ± 5.10)% to (81.92 ± 10.01)% (LUT), (89.33 ± 4.34)% (LUT-STE), and inhibited the cell apoptosis (P < 0.05).After pretreatment with LUT-STE in ARPE cells, the contents of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-PX) in ARPE cells were significantly increased (P < 0.05), the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased.In addition, the vascular-endothelial-cell-growth -factor levels (VEGF) levels were inhibited by 13.61%, 17.39%, respectively, pretreatment with LUT and LUT-STE.Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 protein and caspase-3 protein and up-regulated Bcl-2 /Bax pathway to inhibit H 2 O 2 -induced apoptosis.In summary, the novel delivery LUT-STE had more pronounced inhibitory effect on H 2 O 2 -induced damage in human ARPE cells.