Social Isolation Model: A Noninvasive Rodent Model of Stress and Anxiety

焦虑 抗焦虑药 社会孤立 社会失败 心理学 动物模型 侵略 认知 社会压力 高架加迷宫 临床心理学 医学 发展心理学 神经科学 精神科 内科学
作者
Saki Watanabe,Alzahra Al Omran,Anwen Shao,Jing Liang
出处
期刊:Journal of Visualized Experiments [MyJoVE Corporation]
卷期号: (189) 被引量:3
标识
DOI:10.3791/64567
摘要

Anxiety disorders are one of the leading causes of disability in the United States (US). Current treatments are not always effective and less than 50% of patients achieve full remission. A critical step in developing a novel anxiolytic is to develop and utilize an animal model, such as mice, to study pathological changes and test drug target(s), efficacy, and safety. Current approaches include genetic manipulation, chronic administration of anxiety-inducing molecules, or the administration of environmental stress. These methods, however, may not realistically reflect anxiety induced throughout daily life. This protocol describes a novel anxiety model, which mimics the intentional or unintentional patterns of social isolation in modern life. The social isolation-induced anxiety model minimizes perceived distractions and invasiveness and utilizes wild type C57BL/6 mice. In this protocol, 6- to 8-week-old mice (male and female) are singly housed in opaque cages to visually block the external environment, such as neighboring mice, for 4 weeks. No environmental enrichments (such as toys) are provided, bedding material is reduced by 50%, any treatment of drug is administered as an agar form, and the exposure/handling of the mice is minimized. Socially isolated mice generated using this protocol exhibit greater anxiety-like behavior, aggression, as well as decreased cognition.
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