Temporal and quantitative analysis of the functional expression of Ca2+-permeable AMPA receptors during LTP

长时程增强 AMPA受体 LTP诱导 敌手 海马结构 化学 神经科学 受体 基础(医学) 谷氨酸受体 生物 内分泌学 生物化学 胰岛素
作者
Yoshihiko Wakazono,Ryosuke Midorikawa,Kogo Takamiya
出处
期刊:Neuroscience Research [Elsevier BV]
被引量:1
标识
DOI:10.1016/j.neures.2023.07.002
摘要

In the present study, we attempted to temporally and quantitatively analyze the functional contributions of Ca2+-permeable (CP) α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) during long-term potentiation (LTP) expression using electrophysiological and pharmacological approaches. In hippocampal CA1 neurons, using 1-naphthyl acetyl spermine (NASPM), a CP-AMPAR antagonist, we began by demonstrating that NASPM-sensitive components, probably including the GluA1 homomer, functionally contributed to about 15% of AMPAR-mediated EPSC amplitude in basal conditions. Then, when NASPM was treated at different time points (3-30 min) after LTP induction, it was found that LTP was almost completely impaired at 3 or 10 min but maintained at 20 or 30 min, although its potentiation was reduced. Further temporal and quantitative analysis revealed that the functional expression of CP-AMPARs began increasing approximately 20 min after LTP induction and reached more than twice the basal level at 30 min. These results suggest that CP-AMPARs in the first 3-10 min of LTP might play an important role in LTP maintenance. Moreover, their decay time was also significantly increased at 30 min, suggesting that CP-AMPARs changed not only quantitatively in LTP but also qualitatively.
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