The double‐stranded RNA‐dependent protein kinase PKR negatively regulates the protein expression of IFN‐β induced by RIG‐I signaling

Abstract Retinoic acid‐inducible gene‐I (RIG‐I) is a cytoplasmic RNA sensor that plays an important role in innate immune responses to viral RNAs. Double‐stranded RNA (dsRNA)‐dependent protein kinase (PKR) is a eukaryotic initiation factor 2α (eIF2α) kinase that is initially involved in the responses of the translational machinery to dsRNA. PKR is also thought to play an essential role in antiviral innate immunity. However, the coordinated mechanisms of RIG‐I and PKR that induce the expression of type I interferons (IFNs), essential cytokines involved in antiviral defense, are not completely understood. In this study, we show that PKR negatively participates in the RIG‐I‐mediated induction of IFN‐β expression. Stress granule (SG) formation is crucial to sequester mRNA to prevent aberrant protein synthesis by various stresses. SG formation in response to dsRNA was triggered by a PKR‐mediated antiviral stress response. However, IFN‐β mRNA was not sequestered in the SGs of dsRNA‐treated cells. dsRNA‐induced translational silencing was thought to be PKR dependent. However, our results indicated that some proteins, including IFN‐β, were clearly translated despite PKR‐mediated translational silencing. This study suggests that RIG‐I responds mainly to IFN‐β expression in cells to which non‐self dsRNA is introduced. In addition, PKR negatively regulates IFN‐β protein expression induced by RIG‐I signaling. This may explain the essential role of PKR in fine‐tuning the expression of IFN‐β in RIG‐I‐mediated antiviral immune responses.