生物
电泳迁移率测定
DNA
螺旋(腹足类)
螺旋转动螺旋
生物物理学
DNA结合域
调节器
DNA结合蛋白
基因
分子生物学
转录因子
遗传学
生态学
蜗牛
作者
Xia Fan,Xu Zhang,Yuwei Zhu,Liwen Niu,Maikun Teng,Baolin Sun,Li Xu
出处
期刊:Acta Crystallographica Section D-biological Crystallography
[International Union of Crystallography]
日期:2015-07-31
卷期号:71 (8): 1768-1776
被引量:11
标识
DOI:10.1107/s1399004715010287
摘要
The SaeR/S two-component regulatory system is essential for controlling the expression of many virulence factors in Staphylococcus aureus. SaeR, a member of the OmpR/PhoB family, is a response regulator with an N-terminal regulatory domain and a C-terminal DNA-binding domain. In order to elucidate how SaeR binds to the promoter regions of target genes, the crystal structure of the DNA-binding domain of SaeR (SaeR(DBD)) was solved at 2.5 Å resolution. The structure reveals that SaeR(DBD) exists as a monomer and has the canonical winged helix-turn-helix module. EMSA experiments suggested that full-length SaeR can bind to the P1 promoter and that the binding affinity is higher than that of its C-terminal DNA-binding domain. Five key residues on the winged helix-turn-helix module were verified to be important for binding to the P1 promoter in vitro and for the physiological function of SaeR in vivo.
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