已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

The Antitumor Effect and Mechanism of Total Flavonoids From Coreopsis Tinctoria Nutt (Snow Chrysanthemum) on Lung Cancer Using Network Pharmacology and Molecular Docking

化学 小桶 PI3K/AKT/mTOR通路 细胞凋亡 药理学 蛋白激酶B 信号转导 传统医学 对接(动物) 生物化学 生物 医学 基因表达 基因 护理部 转录组
作者
Yilimire Wufuer,Xu Yang,Luyuan Guo,Kasimujing Aximujiang,Li Zhong,Kurexi Yunusi,Guixia Wu
出处
期刊:Frontiers in Pharmacology [Frontiers Media SA]
卷期号:13 被引量:10
标识
DOI:10.3389/fphar.2022.761785
摘要

Coreopsis tinctoria Nutt (C. tinctoria), also known as Snow Chrysanthemum, is rich in polyphenols and flavonoids. It has important pharmacological effects such as lowering blood lipids, regulating blood glucose, and anti-tumor effect. However, its anti-tumor mechanism has not yet been investigated thoroughly. This study aimed to explore the anti-tumor effect of total flavonoids extracted from C. tinctoria (CTFs) on lung cancer and the possible mechanism. The components of CTFs were analyzed using Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The active components of CTFs were screened according to oral bioavailability (OB) and drug-likeness (DL). Totally, 68 components of CTFs were identified and 23 active components were screened. Network pharmacological analysis on the active components identified 288 potential targets associated with lung cancer. After protein-protein interaction (PPI) network topology analysis, 17 key protein targets including Akt1, MAPK1, TP53, Bcl-2, Caspase-3, Bax, GSK3B and CCND1 were screened. The molecular docking results showed that the active components of CTFs had good binding activity with key targets. GO and KEGG analysis of candidate targets found that the main enrichment was in PI3K/Akt-mediated intrinsic apoptotic pathways. Finally, according to the results of network pharmacology, the potential molecular mechanism of CTFs intervention in lung cancer was validated experimentally in vitro and in vivo. The experimental validation results demonstrated that the antitumor activity of CTFs on lung cancer may be related to inhibiting the PI3K-Akt signaling pathway and activating the mitochondrial-mediated apoptosis pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI

祝大家在新的一年里科研腾飞
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
赘婿应助暗芒采纳,获得10
刚刚
5秒前
Mecury发布了新的文献求助10
6秒前
Mecury完成签到,获得积分10
12秒前
tuanheqi完成签到,获得积分0
15秒前
木木三完成签到,获得积分10
19秒前
称心的语梦完成签到,获得积分10
21秒前
22秒前
mangojuice发布了新的文献求助10
26秒前
我是老大应助wyq采纳,获得10
26秒前
27秒前
29秒前
喵喵完成签到 ,获得积分10
29秒前
科研小白完成签到 ,获得积分10
29秒前
ZXR完成签到,获得积分10
30秒前
五月完成签到,获得积分10
32秒前
33秒前
丁爽发布了新的文献求助10
37秒前
37秒前
eschew完成签到,获得积分10
39秒前
wyq发布了新的文献求助10
40秒前
41秒前
华仔应助丁爽采纳,获得10
43秒前
科研通AI2S应助钟离的摩拉采纳,获得10
44秒前
LuoYixiang完成签到,获得积分10
45秒前
君寻完成签到 ,获得积分10
45秒前
LuoYixiang发布了新的文献求助10
47秒前
小千完成签到 ,获得积分10
52秒前
53秒前
美满一手完成签到,获得积分20
56秒前
Zn0103完成签到 ,获得积分10
57秒前
58秒前
mmm完成签到 ,获得积分10
1分钟前
hwen1998完成签到 ,获得积分10
1分钟前
年轻的冰海完成签到,获得积分10
1分钟前
丁爽完成签到,获得积分20
1分钟前
1分钟前
1分钟前
打打应助悠悠采纳,获得30
1分钟前
A.y.w完成签到,获得积分10
1分钟前
高分求助中
Востребованный временем 2500
Les Mantodea de Guyane 1000
Very-high-order BVD Schemes Using β-variable THINC Method 930
Field Guide to Insects of South Africa 660
The Three Stars Each: The Astrolabes and Related Texts 500
effects of intravenous lidocaine on postoperative pain and gastrointestinal function recovery following gastrointestinal surgery: a meta-analysis 400
The Collected Works of Jeremy Bentham: Rights, Representation, and Reform: Nonsense upon Stilts and Other Writings on the French Revolution 320
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3384333
求助须知:如何正确求助?哪些是违规求助? 2998395
关于积分的说明 8778592
捐赠科研通 2683920
什么是DOI,文献DOI怎么找? 1470072
科研通“疑难数据库(出版商)”最低求助积分说明 679585
邀请新用户注册赠送积分活动 671945