核糖核酸
纳米材料
化学
核酸
纳米技术
内吞作用
内化
小RNA
计算生物学
细胞生物学
生物物理学
细胞
生物
生物化学
材料科学
基因
作者
Xinlian Liu,Ya Wang,Clement Yaw Effah,Longjie Wu,Fei Yu,Jinlan Wei,Guojiang Mao,Yamin Xiong,Leiliang He
出处
期刊:Talanta
[Elsevier]
日期:2022-03-16
卷期号:243: 123377-123377
被引量:12
标识
DOI:10.1016/j.talanta.2022.123377
摘要
Recently, using nanomaterials to enhance the endocytosis capability and sensitivity of probes for RNA imaging in living cells has gotten the attention of many researchers. Nanomaterials, as a reliable alternative to transfection reagents, could prevent nucleic acid probes from being degraded by DNase, and bring them into sub-cellular locations for efficient internalization. Therefore, nanomaterial-based fluorescent probes (NFPs) provide a promising sensing platform to realize in situ RNA detection and imaging, which can reveal the expression of RNA at single cell level and provide large amount of information about RNA spatial localization. Meanwhile, many RNAs are in low abundance in living cells, resulting in difficulty in sensitive detection. Thus, the incorporation of NFPs and signal amplification strategy offers a broader prospect for the detection of RNAs, that have been proven as predominant therapeutic targets or diagnostic biomarkers. Herein, the purpose of our review is to first introduce the general procedure of NFPs used for in situ RNA imaging and how nanomaterials deliver these probes into living cells. Further, we focused on different kinds of nanomaterials that are mainly used for sensitive detection of RNAs and those in low abundance, through different signal read-out modes.
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