Identification of Novel ARF Binding Proteins by Two-Hybrid Screening

The ARF tumor suppressor protects us against cancer through protein-protein interactionsin partially defined p53-dependent and p53-independent pathways. We performed a two-hybridscreen using ARF as bait and present the identification of several new ARF partners that mayregulate its growth inhibitory signaling. The potential physiological roles of these novel ARFbinding proteins in regulating ARF signaling are discussed.