上丘
视网膜
外侧膝状核
神经科学
巨大视网膜神经节细胞
视网膜
视网膜神经节细胞
神经节
视网膜波
视神经
细胞生物学
生物
突触
视网膜神经节细胞
化学
生物化学
作者
Alissa Winzeler,Jack T. Wang
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2013-07-01
卷期号:2013 (7): pdb.top070961-pdb.top070961
被引量:4
标识
DOI:10.1101/pdb.top070961
摘要
Retinal ganglion cells (RGCs) are the neurons that extend axons through the optic nerve, connecting and transmitting information from the retina to the brain. In mammals, RGCs receive information from bipolar and amacrine cells and synapse onto target cells in the lateral geniculate nucleus (LGN) as well as the superior colliculus. Methods for acute purification of RGCs from rodent retina by immunopanning followed by culture in a serum-free medium have facilitated the study of neuronal biology and function in a defined environment. These methods are introduced here, and modifications for achieving optimal RGC purity and culture are described.
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