生发中心
免疫球蛋白类转换
记忆B细胞
生物
B细胞
抗体
亲和力成熟
细胞生物学
同型
基因
等离子体电池
细胞
遗传学
单克隆抗体
作者
Hamish W. King,Nara Orban,John C. Riches,Andrew Clear,Gary Warnes,Sarah A. Teichmann,Louisa K. James
出处
期刊:Science immunology
[American Association for the Advancement of Science (AAAS)]
日期:2021-02-12
卷期号:6 (56)
被引量:159
标识
DOI:10.1126/sciimmunol.abe6291
摘要
Protective humoral memory forms in secondary lymphoid organs where B cells undergo affinity maturation and differentiation into memory or plasma cells. Here, we provide a comprehensive roadmap of human B cell maturation with single-cell transcriptomics matched with bulk and single-cell antibody repertoires to define gene expression, antibody repertoires, and clonal sharing of B cell states at single-cell resolution, including memory B cell heterogeneity that reflects diverse functional and signaling states. We reconstruct gene expression dynamics during B cell activation to reveal a pre-germinal center state primed to undergo class switch recombination and dissect how antibody class-dependent gene expression in germinal center and memory B cells is linked with a distinct transcriptional wiring with potential to influence their fate and function. Our analyses reveal the dynamic cellular states that shape human B cell-mediated immunity and highlight how antibody isotype may play a role during their antibody-based selection.
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