Fibronectin type III domain containing four promotes differentiation of C2C12 through the Wnt/β‐catenin signaling pathway

The FASEB JournalVolume 34, Issue 6 p. 7759-7772 RESEARCH ARTICLEFree to Read Fibronectin type III domain containing four promotes differentiation of C2C12 through the Wnt/β-catenin signaling pathway Jiwei Li, Jiwei Li The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorYanshuang Wang, Yanshuang Wang The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorYan Wang, Yan Wang The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorYunqin Yan, Yunqin Yan The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorHuili Tong, Huili Tong The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorShufeng Li, Corresponding Author Shufeng Li [email protected] The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China Correspondence Shufeng Li, The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang Strict, Harbin, Heilongjiang 150030, China. Email: [email protected]Search for more papers by this author Jiwei Li, Jiwei Li The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorYanshuang Wang, Yanshuang Wang The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorYan Wang, Yan Wang The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorYunqin Yan, Yunqin Yan The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorHuili Tong, Huili Tong The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, ChinaSearch for more papers by this authorShufeng Li, Corresponding Author Shufeng Li [email protected] The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, China Correspondence Shufeng Li, The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang Strict, Harbin, Heilongjiang 150030, China. Email: [email protected]Search for more papers by this author First published: 16 April 2020 https://doi.org/10.1096/fj.201902860RRRCitations: 1Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Fibronectin type III domain containing 4 (FNDC4) belongs to the fibronectin type III domain containing protein family. FNDC5, which is highly homologous to FNDC4, can promote the differentiation of cardiac cells. We aimed to investigate the role of FNDC4 in the differentiation of C2C12 mouse skeletal muscle cells. Western blotting and immunofluorescence analysis showed that FNDC4 gradually increased with the differentiation of C2C12. Muscle injury repair experiments indicated that FNDC4 may promote the repair of injured muscles. When FNDC4 was either overexpressed or knocked down, the expression of desmin and myogenin myogenic marker molecules followed that of FNDC4, suggesting that FNDC4 can influence the differentiation of C2C12. In addition, immunoprecipitation results showed that FNDC4 can interact with the Wnt/β-catenin signaling pathway receptor low-density lipoprotein receptor-related protein 6 (LRP6), and that β-catenin levels in the nucleus decreased after knocking down FNDC4. Exogenous addition of FNDC4 protein could not restore the blocking of differentiation due to inhibition of both Wnt/β-catenin signal transduction and LRP6 activity via the β-catenin inhibitor XAV-939. Overall, our findings indicate that FDNC4 can influence the differentiation of C2C12 by activating Wnt/β-catenin signal transduction. CONFLICT OF INTEREST The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article. Citing Literature Supporting Information Filename Description fsb220511-sup-0001-FigS1.tifTIFF image, 2.8 MB Fig S1 fsb220511-sup-0002-Legend.docxWord document, 14 KB Supplementary Material Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume34, Issue6June 2020Pages 7759-7772 RelatedInformation