Bioremoval of toxic malachite green from water through simultaneous decolorization and degradation using laccase immobilized biochar

孔雀绿 漆酶 化学 核化学 生物炭 色谱法 傅里叶变换红外光谱 吸附 高效液相色谱法 固定化酶 有机化学 化学工程 热解 工程类
作者
Deepshikha Pandey,Achlesh Daverey,Kasturi Dutta,A. Arunachalam
出处
期刊:Chemosphere [Elsevier]
卷期号:297: 134126-134126 被引量:49
标识
DOI:10.1016/j.chemosphere.2022.134126
摘要

In this study, decolorization and degradation of malachite green dye was studied using the laccase immobilized pine needle biochar. Successful immobilization of biochar was achieved by adsorption and confirmed through scanning electron microscopy and energy dispersive X-ray analysis (SEM-EDX), Fourier transform infrared spectroscopy (FTIR). High laccase binding of 64.4 U/g and high immobilization yield of 78.1% was achieved using 4U of enzyme at pH3 and temperature 30 °C. The immobilized laccase retained >50% relative activity in the pH range 2-7, >45% relative activity at 65 °C and >55% relative activity at 4 °C for 4 weeks. The re-usability of immobilized enzyme was checked with 2, 2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) substrate and enzyme retained 53% of its activity after 6 cycles. Immobilized laccase was used for the degradation and decolorization of azo dye malachite green in aqueous solution. More than 85% removal of malachite green dye (50 mg/L) was observed within 5 h. FTIR and high performance liquid chromatography (HPLC) analysis clearly indicated the breakdown of dye and presence of metabolites (leuco malachite green, methanone, [4-(dimethyl amino)pheny]phenyl and 3-dimethyl-phenyl amine) in gas chromatography-mass spectrometry (GC-MS) analysis confirmed the dye degradation. Phytotoxicity analysis indicated that the enzymatic degradation resulted in lesser toxic metabolites than the original dye. Thus, laccase immobilized biochar can be used as an efficient biocatalytic agent to remove dye from water.
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