亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Bioremoval of toxic malachite green from water through simultaneous decolorization and degradation using laccase immobilized biochar

孔雀绿 漆酶 化学 核化学 生物炭 色谱法 傅里叶变换红外光谱 吸附 高效液相色谱法 固定化酶 有机化学 化学工程 热解 工程类
作者
Deepshikha Pandey,Achlesh Daverey,Kasturi Dutta,A. Arunachalam
出处
期刊:Chemosphere [Elsevier]
卷期号:297: 134126-134126 被引量:49
标识
DOI:10.1016/j.chemosphere.2022.134126
摘要

In this study, decolorization and degradation of malachite green dye was studied using the laccase immobilized pine needle biochar. Successful immobilization of biochar was achieved by adsorption and confirmed through scanning electron microscopy and energy dispersive X-ray analysis (SEM-EDX), Fourier transform infrared spectroscopy (FTIR). High laccase binding of 64.4 U/g and high immobilization yield of 78.1% was achieved using 4U of enzyme at pH3 and temperature 30 °C. The immobilized laccase retained >50% relative activity in the pH range 2-7, >45% relative activity at 65 °C and >55% relative activity at 4 °C for 4 weeks. The re-usability of immobilized enzyme was checked with 2, 2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) substrate and enzyme retained 53% of its activity after 6 cycles. Immobilized laccase was used for the degradation and decolorization of azo dye malachite green in aqueous solution. More than 85% removal of malachite green dye (50 mg/L) was observed within 5 h. FTIR and high performance liquid chromatography (HPLC) analysis clearly indicated the breakdown of dye and presence of metabolites (leuco malachite green, methanone, [4-(dimethyl amino)pheny]phenyl and 3-dimethyl-phenyl amine) in gas chromatography-mass spectrometry (GC-MS) analysis confirmed the dye degradation. Phytotoxicity analysis indicated that the enzymatic degradation resulted in lesser toxic metabolites than the original dye. Thus, laccase immobilized biochar can be used as an efficient biocatalytic agent to remove dye from water.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
orixero应助吴彦祖采纳,获得10
17秒前
26秒前
yun发布了新的文献求助10
26秒前
30秒前
31秒前
胖胖猪完成签到,获得积分10
34秒前
科研通AI2S应助葛力采纳,获得10
46秒前
Owen应助科研通管家采纳,获得10
46秒前
53秒前
科研小白完成签到,获得积分10
54秒前
jianghs完成签到,获得积分10
57秒前
58秒前
1分钟前
1分钟前
1分钟前
1分钟前
雪白秋柔完成签到 ,获得积分10
1分钟前
lin完成签到,获得积分10
1分钟前
1分钟前
桐桐应助Zoe采纳,获得10
1分钟前
田様应助吴彦祖采纳,获得10
1分钟前
咖啡续命发布了新的文献求助10
1分钟前
1分钟前
1分钟前
wang完成签到 ,获得积分10
1分钟前
llm发布了新的文献求助10
1分钟前
1分钟前
llm完成签到,获得积分10
1分钟前
Owen应助好想喝奶茶采纳,获得10
1分钟前
2分钟前
benbenca完成签到,获得积分10
2分钟前
2分钟前
fang完成签到,获得积分10
2分钟前
benbenca发布了新的文献求助30
2分钟前
fang发布了新的文献求助10
2分钟前
2分钟前
2分钟前
2分钟前
科研通AI2S应助葛力采纳,获得10
2分钟前
Ava应助科研通管家采纳,获得10
2分钟前
高分求助中
歯科矯正学 第7版(或第5版) 1004
Semiconductor Process Reliability in Practice 1000
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 600
GROUP-THEORY AND POLARIZATION ALGEBRA 500
Mesopotamian divination texts : conversing with the gods : sources from the first millennium BCE 500
Days of Transition. The Parsi Death Rituals(2011) 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3234546
求助须知:如何正确求助?哪些是违规求助? 2880887
关于积分的说明 8217265
捐赠科研通 2548495
什么是DOI,文献DOI怎么找? 1377786
科研通“疑难数据库(出版商)”最低求助积分说明 647999
邀请新用户注册赠送积分活动 623314