生物
影像学
细胞器
领域(数学)
细胞生物学
活体细胞成像
纳米技术
计算生物学
细胞
计算机科学
人工智能
生物化学
材料科学
数学
纯数学
作者
Léia Colin,Raquel Martín-Arevalillo,Simone Bovio,Amélie Bauer,Teva Vernoux,Marie‐Cécile Caillaud,Benoît Landrein,Yvon Jaillais
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2021-09-23
卷期号:34 (1): 247-272
被引量:31
标识
DOI:10.1093/plcell/koab237
摘要
Abstract At the center of cell biology is our ability to image the cell and its various components, either in isolation or within an organism. Given its importance, biological imaging has emerged as a field of its own, which is inherently highly interdisciplinary. Indeed, biologists rely on physicists and engineers to build new microscopes and imaging techniques, chemists to develop better imaging probes, and mathematicians and computer scientists for image analysis and quantification. Live imaging collectively involves all the techniques aimed at imaging live samples. It is a rapidly evolving field, with countless new techniques, probes, and dyes being continuously developed. Some of these new methods or reagents are readily amenable to image plant samples, while others are not and require specific modifications for the plant field. Here, we review some recent advances in live imaging of plant cells. In particular, we discuss the solutions that plant biologists use to live image membrane-bound organelles, cytoskeleton components, hormones, and the mechanical properties of cells or tissues. We not only consider the imaging techniques per se, but also how the construction of new fluorescent probes and analysis pipelines are driving the field of plant cell biology.
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