胶体金
检出限
核酸
生物传感器
DNA
注意事项
线性范围
纳米技术
链霉亲和素
聚合酶链反应
再现性
重组酶聚合酶扩增
核酸定量
材料科学
化学
组合化学
纳米颗粒
色谱法
环介导等温扩增
生物化学
基因
生物素
医学
护理部
作者
Ya Gao,Xilei Deng,Wei Wen,Xun Zhang,Shengfu Wang
标识
DOI:10.1016/j.bios.2016.10.068
摘要
A novel three-dimensional DNA-AuNPs network structure amplification strategy was employed to design a lateral flow biosensor by introducing streptavidin coated gold nanoparticles (Au-SA) in this paper. They act as amplification probes which aggregate numerous gold nanoparticles (AuNPs) on test line by forming a three-dimensional DNA-AuNPs network structure in the presence of target. Sensitive detection of nucleic acid with point-of-care analysis is significant for infectious agent, early diagnosis and treatment of genetic diseases. The use of these particles in rapid ultrasensitive point of care (POC) lateral flow assays lead to a linear range from 0.1 pM to 250 nM with a limit of detection of 0.01 pM without polymerase chain reaction (PCR). The proposed method could increase the sensitivity by 4 orders of magnitudes than traditional sandwich assays labeled with AuNPs. Furthermore, the assay owns good reproducibility and stability, which will prove practical diagnostic applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI