生物高聚物
化学
果胶
多糖
纳米颗粒
动态光散射
化学工程
球状蛋白
热稳定性
乳清蛋白
β-乳球蛋白
色谱法
有机化学
聚合物
结晶学
生物化学
工程类
作者
Owen G. Jones,Uri Lesmes,Paul L. Dubin,David Julian McClements
标识
DOI:10.1016/j.foodhyd.2009.11.003
摘要
Biopolymer nanoparticles can be formed by heating globular protein/polysaccharide mixtures above the thermal denaturation temperature of the protein under pH conditions where the two biopolymers are weakly electrically attracted to each other. In this study, the influence of polysaccharide linear charge density on the formation and properties of these biopolymer nanoparticles was examined. Mixed solutions of globular proteins (β-lactoglobulin) and anionic polysaccharides (high and low methoxyl pectin) were prepared. Micro-electrophoresis, dynamic light scattering, turbidity and atomic force microscopy (AFM) measurements were used to determine the influence of protein-to-polysaccharide mass ratio (r), solution pH, and heat treatment on biopolymer particle formation. Biopolymer nanoparticles (d < 500 nm) could be formed by heating protein–polysaccharide complexes at 83 °C for 15 min at pH 4.75 and r = 2:1 in the absence of added salt. The biopolymer particles formed were then subjected to pH and salt adjustment to determine their stability. The pH stability was greater for β-lactoglobulin-HMP complexes than for β-lactoglobulin-LMP complexes. The addition of 200 mM sodium chloride to heated complexes greatly improved the pH stability of HMP complexes, but decreased the pH stability of LMP complexes. The biopolymer particles formed consisted primarily of β-lactoglobulin, which was probably surrounded by a pectin coating at low pH values. AFM measurements indicated that the biopolymer nanoparticles formed were spheroid in shape. These biopolymer particles may be useful as delivery systems or fat mimetics.
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