细胞生物学
胞浆
线粒体
细胞器
内膜转移酶
生物发生
线粒体内膜
小泡
外膜转位酶
膜
化学
生物物理学
生物
细胞室
细菌外膜
线粒体膜转运蛋白
生物化学
细胞
大肠杆菌
基因
酶
作者
Zachary N. Wilson,Matt West,Alyssa M. English,Greg Odorizzi,Adam L. Hughes
标识
DOI:10.1083/jcb.202307035
摘要
Preserving the health of the mitochondrial network is critical to cell viability and longevity. To do so, mitochondria employ several membrane remodeling mechanisms, including the formation of mitochondrial-derived vesicles (MDVs) and compartments (MDCs) to selectively remove portions of the organelle. In contrast to well-characterized MDVs, the distinguishing features of MDC formation and composition remain unclear. Here, we used electron tomography to observe that MDCs form as large, multilamellar domains that generate concentric spherical compartments emerging from mitochondrial tubules at ER-mitochondria contact sites. Time-lapse fluorescence microscopy of MDC biogenesis revealed that mitochondrial membrane extensions repeatedly elongate, coalesce, and invaginate to form these compartments that encase multiple layers of membrane. As such, MDCs strongly sequester portions of the outer mitochondrial membrane, securing membrane cargo into a protected domain, while also enclosing cytosolic material within the MDC lumen. Collectively, our results provide a model for MDC formation and describe key features that distinguish MDCs from other previously identified mitochondrial structures and cargo-sorting domains.
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