The electron transport chain of Shewanella oneidensis MR-1 can operate bidirectionally to enable microbial electrosynthesis

舍瓦内拉 电子传输链 阴极 电合成 氧化还原 化学 电子转移 电子受体 化学渗透 呼吸链 微生物燃料电池 阳极 生物物理学 光化学 化学工程 生物化学 无机化学 电化学 生物 电极 细菌 线粒体 ATP合酶 物理化学 遗传学 工程类
作者
Kathryne C. Ford,Michaela A. TerAvest
出处
期刊:Applied and Environmental Microbiology [American Society for Microbiology]
卷期号:90 (1) 被引量:1
标识
DOI:10.1128/aem.01387-23
摘要

ABSTRACT Extracellular electron transfer is a process by which bacterial cells can exchange electrons with a redox-active material located outside of the cell. In Shewanella oneidensis , this process is natively used to facilitate respiration using extracellular electron acceptors such as Fe(III) or an anode. Previously, it was demonstrated that this process can be used to drive the microbial electrosynthesis (MES) of 2,3-butanediol (2,3-BDO) in S. oneidensis exogenously expressing butanediol dehydrogenase (BDH). Electrons taken into the cell from a cathode are used to generate NADH, which in turn is used to reduce acetoin to 2,3-BDO via BDH. However, generating NADH via electron uptake from a cathode is energetically unfavorable, so NADH dehydrogenases couple the reaction to proton motive force. We therefore need to maintain the proton gradient across the membrane to sustain NADH production. This work explores accomplishing this task by bidirectional electron transfer, where electrons provided by the cathode go to both NADH formation and oxygen (O 2 ) reduction by oxidases. We show that oxidases use trace dissolved oxygen in a microaerobic bioelectrical chemical system (BES), and the translocation of protons across the membrane during O 2 reduction supports 2,3-BDO generation. Interestingly, this process is inhibited by high levels of dissolved oxygen in this system. In an aerated BES, O 2 molecules react with the strong reductant (cathode) to form reactive oxygen species, resulting in cell death. IMPORTANCE Microbial electrosynthesis (MES) is increasingly employed for the generation of specialty chemicals, such as biofuels, bioplastics, and cancer therapeutics. For these systems to be viable for industrial scale-up, it is important to understand the energetic requirements of the bacteria to mitigate unnecessary costs. This work demonstrates sustained production of an industrially relevant chemical driven by a cathode. Additionally, it optimizes a previously published system by removing any requirement for phototrophic energy, thereby removing the additional cost of providing a light source. We also demonstrate the severe impact of oxygen intrusion into bioelectrochemical systems, offering insight to future researchers aiming to work in an anaerobic environment. These studies provide insight into both the thermodynamics of electrosynthesis and the importance of the bioelectrochemical systems’ design.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
杨杨杨完成签到,获得积分10
1秒前
shanshan发布了新的文献求助10
1秒前
1秒前
ding应助科研通管家采纳,获得10
1秒前
思源应助科研通管家采纳,获得10
1秒前
1秒前
今后应助科研通管家采纳,获得10
2秒前
科研通AI6应助科研通管家采纳,获得30
2秒前
科研通AI5应助科研通管家采纳,获得10
2秒前
烟花应助科研通管家采纳,获得10
2秒前
FashionBoy应助科研通管家采纳,获得10
2秒前
小马甲应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
ding应助科研通管家采纳,获得10
2秒前
2秒前
文静的天蓝完成签到,获得积分10
2秒前
NexusExplorer应助科研通管家采纳,获得10
2秒前
陈早早完成签到,获得积分10
2秒前
2秒前
赘婿应助科研通管家采纳,获得10
2秒前
无花果应助科研通管家采纳,获得10
2秒前
香蕉觅云应助科研通管家采纳,获得10
2秒前
HahaNana应助科研通管家采纳,获得20
2秒前
打打应助科研通管家采纳,获得10
3秒前
完美世界应助科研通管家采纳,获得10
3秒前
我是老大应助科研通管家采纳,获得10
3秒前
打打应助科研通管家采纳,获得10
3秒前
深情安青应助科研通管家采纳,获得30
3秒前
不想干活应助科研通管家采纳,获得10
3秒前
3秒前
上官若男应助科研通管家采纳,获得10
3秒前
orixero应助科研通管家采纳,获得10
3秒前
木日完成签到,获得积分10
3秒前
3秒前
Orange应助科研通管家采纳,获得10
3秒前
3秒前
3秒前
科目三应助kmkz采纳,获得30
4秒前
明亮寒安完成签到 ,获得积分10
4秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
网络安全 SEMI 标准 ( SEMI E187, SEMI E188 and SEMI E191.) 1000
计划经济时代的工厂管理与工人状况(1949-1966)——以郑州市国营工厂为例 500
INQUIRY-BASED PEDAGOGY TO SUPPORT STEM LEARNING AND 21ST CENTURY SKILLS: PREPARING NEW TEACHERS TO IMPLEMENT PROJECT AND PROBLEM-BASED LEARNING 500
Why America Can't Retrench (And How it Might) 400
Two New β-Class Milbemycins from Streptomyces bingchenggensis: Fermentation, Isolation, Structure Elucidation and Biological Properties 300
Modern Britain, 1750 to the Present (第2版) 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 物理化学 基因 催化作用 遗传学 冶金 电极 光电子学
热门帖子
关注 科研通微信公众号,转发送积分 4614444
求助须知:如何正确求助?哪些是违规求助? 4018649
关于积分的说明 12439260
捐赠科研通 3701425
什么是DOI,文献DOI怎么找? 2041187
邀请新用户注册赠送积分活动 1073927
科研通“疑难数据库(出版商)”最低求助积分说明 957600