Effect of Dieda Ointment on Bone Morphogenetic Protein-2/Suppressor of Mothers Against Decapentaplegic 1/Runt-Related Transcription Factor 2/Sp7 Transcription Factor Pathway for Fracture Healing in Rats

Dieda ointment, a Chinese medicine, has been widely used in the clinical treatment of fracture after years of clinical research to prove that it has significant effects on healing injury and relieving pain, reinforcing tendons and setting bones. However, the mechanism of its effects on fracture healing is still unclear. The purpose of this study was to explore the mechanism of Dieda ointment in promoting fracture healing. The materials and methods explored were used to establish a fracture model of Sprague-Dawley rats, which was externally applied with Dieda ointment for 1, 2 and 4 w and imaging examination of the fracture site was performed. Histological examination was also performed. Western blotting and quantitative reverse transcription polymerase chain reaction were used to detect the expression of proteins and its messenger ribonucleic acid. We found that Dieda ointment can effectively accelerate callus formation at tibia fractures in rats and increase the messenger ribonucleic acid and protein expression levels of bone morphogenetic protein-2, suppressor of mothers against decapentaplegic 1, runt-related transcription factor 2 and Sp7 transcription factor, regulate bone formation and promote fracture healing. The study on the mechanism of Dieda ointment promoting fracture healing, not only provides theoretical and experimental basis for the subsequent study of Dieda ointment.