Green tea hydroxycinnamoylated catechins extend lifespan and attenuate Aβ-induced paralysis of Caenorhabditis elegans via anti-oxidation and peptides dis-aggregation

Hydroxycinnamoylated catechins (HCCs) could attenuate H2O2 or β-amyloid peptides (Aβ) induced damage in neural cells, thus having anti-Alzheimer's disease (AD) potential. Longjing43 green tea harvested in summer and autumn (LJ43-SA) has high HCCs content. We explored the anti-AD potential of the LJ43-SA together with common catechins and HCCs in green tea. Transgenic Caenorhabditis elegans (C. elegans) CL4176, CL2331, and CL2355 were used to explore the anti-AD effects. LJ43-SA prolonged the lifespan of paralytic nematodes and delayed the occurrence of paralysis. Tea catechins prolonged the lifespan (100 μM epigallocatechin 3-O-p-coumaroate (EGC-pC), 44% promotion) and healthspan of paralytic C. elegans, improving stress resistance, reducing ROS levels, MDA contents and Aβ deposition, enhancing GSH-Px and SOD activities, reinstating benzaldehyde chemotaxis (100 μM EGC-pC, 270% promotion), reducing 5-HT sensitivity (100 μM EGC-pC, 231% promotion), and alleviating glutamate-induced neurotoxicity (100 μM EGC-pC, 184% promotion). These results suggest that green tea extract and HCCs have anti-AD potential.