Vibrio amylolyticus sp. nov. and Vibrio gelatinilyticus sp. nov., two marine bacteria isolated from surface seawater of Qingdao

Two Gram-stain-negative, oxidase-positive, facultative anaerobic and rod-shaped motile bacteria, designated strains ZSDZ34 and ZSDE26, were isolated from offshore surface seawater collected near Qingdao. Phylogenetic analysis based on 16S rRNA gene sequences placed ZSDE26 T and ZSDZ34 T within the genus Vibrio , family Vibrionaceae , class Gammaproteobacteria . Strain ZSDE26 T was most closely related to Vibrio gallaecicus VB 8.9 T with 97.3 % sequence similarity, whereas ZSDZ34 T was most closely related to Vibrio aestuarianus subsp. cardii DSM 109723 T with 97.8 % sequence similarity. Strain ZSDE26 T grew with 1–5 % (w/v) NaCl (optimum, 4 %), at 16–28 °C (optimum, 28 °C) and at pH 6.0–9.0 (optimum, pH 7.0). Growth of strain ZSDZ34 T occurred with 1–6 % (w/v) NaCl (optimum, 3 %), at 16–37 °C (optimum, 28 °C) and at pH 6.0–9.0 (optimum, pH 7.0). Both strains shared the same major fatty acid components (more than 10 % of total fatty acids) of summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ), summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ) and C 16 : 0 . Additionally, strain ZSDZ34 T contained a higher proportion of iso-C 16 : 0 . The DNA G+C contents of strains ZSDE26 T and ZSDZ34 T were 42.8 and 44.5 mol%, respectively. On the basis of the results of polyphasic analysis, ZSDE26 T and ZSDZ34 T are considered to represent novel species within the genus Vibrio , for which the names Vibrio amylolyticus sp. nov. (type strain, ZSDE26 T =KCTC 82890 T =MCCC 1K06290 T ) and Vibrio gelatinilyticus sp. nov. (type strain, ZSDZ34 T =KCTC 82888 T =MCCC 1K06292 T ) are proposed, respectively.