Identification and verification of m6A-related miRNAs correlated with prognosis and immune microenvironment in colorectal cancer

小RNA 结直肠癌 医学 肿瘤科 免疫系统 弗雷明翰风险评分 内科学 肿瘤微环境 接收机工作特性 生物信息学 癌症 计算生物学 疾病 基因 生物 免疫学 遗传学
作者
Xinze Qiu,Da Chen,Shanpei Huang,Ni Chen,Jiangni Wu,Shengmei Liang,Peng Peng,Mengbin Qin,Jiean Huang,Shiquan Liu
出处
期刊:Medicine [Wolters Kluwer]
卷期号:102 (46): e35984-e35984
标识
DOI:10.1097/md.0000000000035984
摘要

It's well known that N6-methyladenosine (m6A) modification is the most abundant modification in multiple RNA species. miRNAs play important roles in m6A modification and are closely related with occurrence and development of colorectal cancer (CRC). Thus, the aim of this study was to identify the prognostic value of m6A-related miRNAs and explore the correlation between the miRNAs and immune microenvironment in CRC. The differentially expressed m6A regulators and differentially expressed miRNAs between CRC tissues and adjacent normal tissues were identified based on TCGA dataset, and the m6A-related miRNAs were screened. The CRC patients from TCGA were randomized (1:1) into training set and validation set, and the risk score was established in the training set. Next, risk score was verified in the validation set and GSE92928 from GEO datasets. Besides, the relationship among tumor mutational burden, immune microenvironment and risk score were analyzed. What's more, RT-qPCR were used to explore the expression levels of the miRNAs in risk score between SW480 and SW620. A total of 29 m6A-related miRNAs were screened out, and a 5-differentially expressed miRNAs risk score was established. Kaplan-Meier analysis and ROC curves revealed the risk score could predict the prognosis of CRC, accurately. Similarly, the patients in the high-risk group had shorter overall survival in GSE92928. The risk score was relevant with the tumor mutational burden and immune infiltration, and the expression of HAVCR2 was significant difference between 2 risk groups. The expression levels of miR-328-3p, miR-3934-5p, miR-664b-5p and miR-3677-3p were down-regulated in SW620 compared with SW480, only the expression level of miR-200c-5p was up-regulated in SW620. The findings provided the new insights into the correlation between miRNAs and m6A regulators. The m6A-related miRNAs could predict the prognosis of CRC and provide the valuable information of immunotherapy in CRC patients.
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