肌成纤维细胞
细胞生物学
伤口愈合
芦荟
人口
化学
山奈酚
槲皮素
药理学
生物
生物化学
免疫学
医学
病理
微生物学
纤维化
环境卫生
抗氧化剂
作者
Orlando Ramírez,Florencia Pomareda,Belén Olivares,Y-C. Huang,Gabriela Zavala,Javiera Carrasco-Rojas,Simón Álvarez,Camila Leiva‐Sabadini,Valeria Susana Hidalgo,Pablo Romo,Matías Agustín Sánchez,A. F. Vargas,Jessica Martínez,Sebastian Aguayo,Christian Schuh
出处
期刊:Phytomedicine
[Elsevier]
日期:2024-01-01
卷期号:122: 155108-155108
被引量:3
标识
DOI:10.1016/j.phymed.2023.155108
摘要
Aloe vera (AV) is a medicinal plant, most known for its beneficial effects on a variety of skin conditions. Its known active compounds include carbohydrates and flavonoids such as quercetin and kaempferol, among others. In the past decade, plant nanovesicles (NVs) have gained considerable interest as interkingdom communicators, presenting an opportunity for clinical standardization of natural products. In this study, we aimed to assess the potential of AVpNVs for the treatment of burn wounds. AVpNVs were isolated and characterized regarding vesicle yield (nanoparticle tracking analysis) and structure (transmission electron microscopy and atomic force microscopy), as well as their protein content with proteomics. We assessed key characteristics for treating burn wounds in vitro, such as the anti-inflammatory potential in LPS-stimulated macrophages and keratinocytes, and the effect of AVpNVs on myofibroblast differentiation and contraction. AVpNVs presented a homogenous NV population, vesicular shape, and NV-associated protein markers. AVpNVs significantly decreased the secretion of pro-inflammatory cytokines TNFα, IL-1β, and IL-6. Furthermore, AVpNVs inhibited myofibroblast differentiation and significantly decreased their contractile potential in collagen matrices. Observed effects were linked to proteins identified in the isolates through proteomics analysis. AVpNVs displayed characteristics as an inflammatory modulator, while simultaneously diminishing myofibroblast differentiation and contraction. Novel strategies for burn wound treatment seek to decrease scarring on a cellular and molecular level in the early stages of wound healing, which makes AVpNVs a promising candidate for future plant-vesicle-based treatments.
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