生物
突触小泡
复印件
转运蛋白
舱室(船)
小泡
突触蛋白
接吻奔跑融合
囊泡转运蛋白
分子马达
囊泡融合
高尔基体
活动区
细胞生物学
生物物理学
生物化学
内质网
分泌途径
膜
海洋学
地质学
作者
Hua Qiu,Xiandeng Wu,Xiaoli Ma,Shulin Li,Qixu Cai,Marcelo Ganzella,Liang Ge,Hong Zhang,Mingjie Zhang
出处
期刊:Cell
[Elsevier]
日期:2024-04-01
卷期号:187 (9): 2175-2193.e21
被引量:5
标识
DOI:10.1016/j.cell.2024.03.003
摘要
Summary
In addition to long-distance molecular motor-mediated transport, cellular vesicles also need to be moved at short distances with defined directions to meet functional needs in subcellular compartments but with unknown mechanisms. Such short-distance vesicle transport does not involve molecular motors. Here, we demonstrate, using synaptic vesicle (SV) transport as a paradigm, that phase separation of synaptic proteins with vesicles can facilitate regulated, directional vesicle transport between different presynaptic bouton sub-compartments. Specifically, a large coiled-coil scaffold protein Piccolo, in response to Ca2+ and via its C2A domain-mediated Ca2+ sensing, can extract SVs from the synapsin-clustered reserve pool condensate and deposit the extracted SVs onto the surface of the active zone protein condensate. We further show that the Trk-fused gene, TFG, also participates in COPII vesicle trafficking from ER to the ER-Golgi intermediate compartment via phase separation. Thus, phase separation may play a general role in short-distance, directional vesicle transport in cells.
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