过氧化氢酶
超氧化物歧化酶
光合作用
脂质过氧化
转录组
过氧化物酶
活性氧
抗氧化剂
转基因
盐度
叶绿素
土壤盐分
叶绿体
生物
化学
生物化学
基因
植物
酶
基因表达
生态学
作者
Shuaijun Zhuang,Zhaoyou Yu,Jia Li,Sheng Wang,Chunxia Zhang
出处
期刊:Tree Physiology
[Oxford University Press]
日期:2024-09-04
标识
DOI:10.1093/treephys/tpae113
摘要
Abstract None declared.Conflict of interestSoil salinization has become a global problem and high salt concentration in soil negatively affects plant growth. In our previous study, we found that overexpression of PsAMT1.2 from Populus simonii could improve the salt tolerance of poplar, but the physiological and molecular mechanism was not well understood. To explore the regulation pathway of PsAMT1.2 in salt tolerance, we investigated the morphological, physiological, and transcriptome differences between the PsAMT1.2 overexpression transgenic poplar and the wild type (WT) under salt stress. The PsAMT1.2 overexpression transgenic poplar showed better growth with increased net photosynthetic rate and higher chlorophyll content compared with WT under salt stress. The overexpression of PsAMT1.2 increased the catalase, superoxide dismutase, peroxidase, ascorbate peroxidase activities and therefore probably enhanced the reactive oxygen species clearance ability, which also reduced the degree of membrane lipid peroxidation under salt stress. Meanwhile, the PsAMT1.2 overexpression transgenic poplar maintained a relatively high K+/Na+ ratio under salt stress. RNA-seq analysis indicated that PsAMT1.2 might improve plant salt tolerance by regulating pathways related to the photosynthetic system, chloroplast structure, antioxidant activity, and anion transport. Among the 1056 differentially expressed genes, genes related to photosystemIand photosystemIIwere up-regulated and genes related to chloride channel protein-related were down-regulated. The result of the present study would provide new insight into regulation mechanism of PsAMT1.2 in improving salt tolerance of poplar.
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