亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Sperm induce macrophage extracellular trap formation via phagocytosis-dependent mechanism

细胞外 吞噬作用 巨噬细胞 精子 生物 中性粒细胞胞外陷阱 细胞生物学 精子无力症 精子活力 运动性 免疫学 炎症 生物化学 男性不育 不育 体外 怀孕 遗传学 植物
作者
Chuncheng Lu,Zhao Wu,Hongbin Gao,Haiyuan Li,Renbin Deng,Ning Luo,Shipeng Fan,Xi Li,Danpeng He,Hui Zhao
出处
期刊:Biology of Reproduction [Oxford University Press]
卷期号:109 (3): 319-329 被引量:1
标识
DOI:10.1093/biolre/ioad068
摘要

Infertility is a public health concern worldwide. Asthenozoospermia is a common cause of male infertility and is characterized by decreased motility. Sperm motility ensures that sperm migrate to complete fertilization. Macrophages are an essential component of innate immunity in the female reproductive tract. Macrophage extracellular traps are induced by various microorganisms to capture and mediate the clearance of microorganisms. The relationship between sperm and macrophage extracellular traps is unclear. The human monocyte leukemia (THP-1) cells differentiated by phorbol myristate acetate (PMA) are widely used as surrogate of human macrophages. This study investigated sperm-induced macrophage extracellular trap formation and clarified some of the mechanisms affecting macrophage extracellular trap production. Sperm-induced macrophage extracellular traps were visualized and components of macrophage extracellular traps were identified by immunofluorescence analyses and scanning electron microscopy. By inhibiting macrophage extracellular trap production and macrophage phagocytosis, the relationship between macrophage phagocytosis and macrophage extracellular trap production was analyzed. Sperm could trigger PMA-differentiated THP-1 macrophages to produce extracellular traps. Sperm-triggered macrophage extracellular traps are dependent on phagocytosis and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Sperm from asthenozoospermia donors are more likely to be phagocytosed by macrophages than sperm from healthy donors, which induce more macrophage extracellular trap release. These data confirm the phenomenon and partial mechanism of sperm-induced macrophage extracellular trap formation in vitro. These may partly provide evidence to explain the mechanisms of clearing abnormally morphological or hypomotile sperm in the female reproductive tract and the rationale for the decreased probability of successful fertilization in asthenozoospermia.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
SciGPT应助科研通管家采纳,获得10
5秒前
隐形曼青应助江彪采纳,获得10
17秒前
25秒前
江彪发布了新的文献求助10
30秒前
32秒前
一剑白完成签到 ,获得积分10
44秒前
。。完成签到 ,获得积分10
1分钟前
charliechen完成签到 ,获得积分10
1分钟前
传奇完成签到 ,获得积分10
1分钟前
过时的柚子完成签到,获得积分10
1分钟前
2分钟前
NexusExplorer应助科研通管家采纳,获得10
2分钟前
NexusExplorer应助科研通管家采纳,获得10
2分钟前
白华苍松发布了新的文献求助10
2分钟前
JamesPei应助andrele采纳,获得10
2分钟前
2分钟前
2分钟前
2分钟前
充电宝应助小鲤鱼在睡觉采纳,获得10
2分钟前
小鲤鱼在睡觉完成签到,获得积分10
2分钟前
3分钟前
andrele发布了新的文献求助30
3分钟前
CHL完成签到 ,获得积分10
3分钟前
情怀应助科研通管家采纳,获得10
4分钟前
科研通AI2S应助科研通管家采纳,获得10
4分钟前
4分钟前
andrele发布了新的文献求助10
4分钟前
6分钟前
脑洞疼应助科研通管家采纳,获得20
6分钟前
华仔应助于是乎采纳,获得10
6分钟前
iehaoang完成签到 ,获得积分10
7分钟前
h0jian09完成签到,获得积分10
7分钟前
李爱国应助科研通管家采纳,获得10
8分钟前
搜集达人应助科研通管家采纳,获得10
8分钟前
JamesPei应助科研通管家采纳,获得10
8分钟前
andrele发布了新的文献求助10
8分钟前
CCC完成签到,获得积分10
8分钟前
可乐完成签到,获得积分10
8分钟前
从容芮应助CCC采纳,获得10
8分钟前
可乐发布了新的文献求助10
9分钟前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3137011
求助须知:如何正确求助?哪些是违规求助? 2787960
关于积分的说明 7784196
捐赠科研通 2444060
什么是DOI,文献DOI怎么找? 1299705
科研通“疑难数据库(出版商)”最低求助积分说明 625497
版权声明 600997