砷
砷酸盐
杜氏盐藻
莱茵衣藻
化学
磷酸盐
植物修复
砷毒性
环境化学
亚砷酸盐
伊乐藻
藻类
生物
水生植物
植物
生物化学
重金属
生态学
基因
突变体
有机化学
水生植物
作者
Yimei Xi,Baoyu Han,Fantao Kong,Tingting You,Ran Bi,Xiangfeng Zeng,Shaofeng Wang,Yongfeng Jia
标识
DOI:10.1016/j.jhazmat.2023.132130
摘要
Arsenate (AsV) is a predominant arsenic contaminant in aerobic water. Microalgae have been recently used in the phytoremediation of arsenic-contaminated water. However, the amount of AsV uptake in microalgae is limited, which hinders the application of microalgae in arsenic-contaminated water treatment. Here, we found that the expression of a novel phosphate transporter DsPht1 in Dunaliella salina was highly upregulated after AsV exposure. Fluorescent protein-tagging analysis showed the plasma membrane location of DsPht1. Furthermore, DsPht1 was overexpressed in a model microalga Chlamydomonas reinhardtii. The DsPht1 transgenetic lines accumulated up to 6.4-fold higher total arsenic than the untransformed line, and the AsV amount in total arsenic increased by 8.3-fold. Moreover, the organoarsenic content was also higher in the transgenetic lines. Overall, the DsPht1 transformants generated in this study increased arsenate uptake and transformation, which are promising for the effective phytoremediation of arsenic-contaminated water.
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