肺炎
病菌
医学
支气管肺泡灌洗
社区获得性肺炎
病因学
重症监护室
内科学
DNA测序
临床诊断
生物
儿科
免疫学
肺
遗传学
DNA
作者
Xin Zhao,Yizhi Ge,Yuwei Zhang,Wenjie Zhang,Haiping Hu,L Li,Tong Sha,Zhenhua Zeng,Wenlin Feng,ZhongQing Chen
摘要
Next-generation sequencing of the metagenome (mNGS) is increasingly used in pathogen diagnosis for infectious diseases due to its short detection time. The time for Oxford Nanopore Technologies (ONT) sequencing-based etiology detection is further shortened compared with that of mNGS, but only a few studies have verified the time advantage and accuracy of ONT sequencing for etiology diagnosis. In 2022, a study confirmed that there was no significant difference in sensitivity and specificity between ONT and mNGS in suspected community-acquired pneumonia patients, which there was no clinical study verified in patients with SHAP.From October 24 to November 20, 2022, 10 patients with severe hospital-acquired pneumonia (SHAP) in the Nanfang Hospital intensive care unit (ICU) were prospectively enrolled. Bronchoalveolar lavage fluid (BALF) was collected for ONT sequencing, mNGS, and traditional culture. The differences in pathogen detection time and diagnostic agreement among ONT sequencing, mNGS, traditional culture method, and clinical composite diagnosis were compared.Compared with mNGS and the traditional culture method, ONT sequencing had a significant advantage in pathogen detection time (9.6±0.7 h versus 24.7±2.7 h versus 132±58 h, P <0.05). The agreement rate between ONT sequencing and the clinical composite diagnosis was 73.3% (kappa value=0.737, P <0.05).ONT sequencing has a potential advantage for rapidly identifying pathogens.
科研通智能强力驱动
Strongly Powered by AbleSci AI