肌上皮细胞
类有机物
唾液腺
胚胎干细胞
导管细胞
间充质
生物
干细胞
细胞生物学
上皮
成体干细胞
内分泌学
胰腺
间充质干细胞
免疫学
免疫组织化学
遗传学
生物化学
基因
作者
Junichi Tanaka,Kenji Mishima
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 247-255
被引量:3
标识
DOI:10.1007/978-1-0716-1979-7_16
摘要
Salivary glands are exocrine glands composed of several cell types, including the ductal, acinar, and basal/myoepithelial cells. They play important roles in maintaining oral homeostasis and health. During early murine development, the salivary glands, which arise as epithelial buds, are produced from primitive oral epithelia through an interaction between the oral epithelium and mesenchyme.We recently reported that salivary gland organoids can be generated from mouse embryonic stem cells (ESCs). We recapitulated the process of embryonic salivary gland development using an organoid culture system. The mouse ESC-derived salivary gland organoids consisted of acinar-, ductal-, and myoepithelial-like cells. In this chapter, we describe a protocol for differentiating salivary gland organoids from ESCs.
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