任天堂
生物发光成像
间充质干细胞
癌症研究
移植
干细胞
医学
肺纤维化
病理
生物医学工程
纤维化
特发性肺纤维化
荧光素酶
化学
肺
细胞生物学
外科
生物
内科学
转染
生物化学
基因
作者
Xiaodi Li,Chenggong Yu,Hongying Bao,Zhongjin Chen,Xiaoyun Liu,Jie Huang,Zhijun Zhang
标识
DOI:10.1016/j.nano.2022.102517
摘要
Mesenchymal stem cells (MSCs) are promising in idiopathic pulmonary fibrosis (IPF) therapy. However, low survival rate and ambiguous behavior of MSCs after transplantation impede their clinical translation. To this end, we have developed a new strategy to improve the survival rate and monitor the behavior of the transplanted MSCs simultaneously. In our strategy, nintedanib, a tyrosine kinase inhibitor, is employed to protect the human MSCs (hMSCs) from excessive oxidative stress responses and inflammatory environment in the damaged lung. Moreover, by labeling of the transplanted hMSCs with a computed tomography (CT) nanotracer, Au nanoparticles functionalized with polyethylenimine (PEI) and polyethylene glycol (PEG) (Au@PEI@PEG), in combination with red-emitting firefly luciferase (RfLuc), in vivo CT/bioluminescence (BL) dual-modal imaging tracking of the location, distribution, and survival of the transplanted hMSCs in presence of nintedanib were achieved, which facilitates the profound understanding of the role the stem cells play in IPF therapy.
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