Citrinin impairs pig oocyte maturation by inducing oxidative stress and apoptosis

Abstract Citrinin (CTN) is a polyketide mycotoxin produced by several strains of Penicillium , Monascus , and Aspergillus . While CTN poses various toxic effects on the female reproductive system in animals, its direct effects on germ cell development are unclear. This study aimed to evaluate the effects of increasing concentrations of CTN (0,20,40,80,100 μM) on porcine oocyte in vitro maturation. Our results indicate that CTN supplementation inhibited polar body extrusion in a dose-dependent manner. Actin and spindle assembly were also disrupted after treatment, indicating that CTN affects the cytoskeleton of porcine oocytes. Oxidative stress and apoptosis were observed under CTN treatment to explore the cause of meiotic maturation failure in porcine oocytes. The results showed that reactive oxygen species levels, cathepsin B activity, and caspase-3 activity were increased in the treated group, indicating that CTN induced oxidative stress and apoptosis. In conclusion, CTN exposure could reduce porcine oocyte maturation by affecting cytoskeletal dynamics, oxidative stress, and apoptosis. • CTN exposure inhibited porcine oocyte maturation. • CTN exposure disrupted the cytoskeleton of porcine oocyte. • CTN exposure induced oxidative stress in porcine oocyte. • CTN exposure caused apoptosis in porcine oocytes.