Effect of matrine combined with cisplatin on drug sensitivity and TrkB expression in SH-SY5Y cells

Objective To explore the effects of matrine(Ma) on survival and apoptosis of neuroblastoma cell line SH-SY5Y,reduction of drug-resistance of cisplatin(CDDP) and its potential mechanism.Methods SH-SY5Y cells were treated with all-trans-retinoic acid(ATRA),brain-derived neurotrophic factor,CDDP and Ma respectively.Trials were divided into following groups: control group,ATRA induced cisplatin intervention group,cisplatin intervention group,Ma intervention group,and Ma combined with cisplatin intervention group.MTT colorimetric assay was performed to exam the survival rate of SH-SY5Y cells treated with Ma and CDDP.Apoptosis rate of SY5Y was measured by flow cytometry Ma.Western-blot analysis was employed to measure the p-TrkB expression.Results There was significant difference in survival and apoptosis of SH-SY5Y cells between each group and control group except the ATRA induced cisplatin intervention group(P 0.05).Significant difference was also found between cisplatin intervention group and ATRA induced cisplatin intervention group(P 0.05).The survival rates and apoptosis of SH-SY5Y cells was statistically significant between Ma group and Ma combined with cisplation group(P 0.05);and also between the Ma joint cisplation group and cisplation intervention group(P 0.05);There was no significant difference in survival and apoptosis of SH-SY5Y cells between Ma(0.5 mg / ml) intervention group and ATRA induced cisplatin group(P 0.05).No significant difference was observed in p-TrkB expression of SH-SY5Y cells among groups(P 0.05).Conclusions Ma can induce cell apoptosis and inhibit the growth of the SH-SY5Y cells,meanwhile increase the sensibility of chemotherapy of cisplatin but this effect is not correlated to the TrkB /BDNF signal transduction.