Regulation of preimplantation embryo development by brain-derived neurotrophic factor

原肌球蛋白受体激酶B 胚泡 生物 脑源性神经营养因子 trk受体 神经营养素 神经营养因子 胚胎 原肌球蛋白受体激酶A 胚胎发生 自分泌信号 旁分泌信号 外域 细胞生物学 内分泌学 内科学 受体 遗传学 医学
作者
Kazuhiro Kawamura,Nanami Kawamura,Jun Fukuda,Jin Kumagai,Aaron J. W. Hsueh,Toshinobu Tanaka
出处
期刊:Developmental Biology [Elsevier]
卷期号:311 (1): 147-158 被引量:95
标识
DOI:10.1016/j.ydbio.2007.08.026
摘要

Hormonal factors secreted by embryos and reproductive tracts are important for successful development of preimplantation embryos. We found expression of brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5) transcripts at its highest levels in the blastocyst stages. The transcripts for their receptor, TrkB, were detectable throughout the early embryonic stages with an increase after the early blastocyst stage. Both BDNF and TrkB are expressed in trophectoderm cells, whereas ligand-binding studies indicated specific binding of BDNF to trophectoderm cells. Furthermore, BDNF and NT-4/5 were produced in pregnant oviducts and uteri. Treatment with BDNF promoted the development of two-cell-stage embryos into blastocysts showing increased proliferation and decreased apoptosis. The effects of BDNF were blocked by the TrkB ectodomain or a Trk receptor inhibitor, K252a. Studies using specific inhibitors demonstrated the roles of the PI3K, but not the ERK, pathway in mediating BDNF actions. Under high-density embryo cultures, treatment with the TrkB ectodomain or K252a alone also inhibited embryonic development and survival, suggesting potential autocrine actions of BDNF produced by the embryo. In vivo experiments further demonstrated that K252a treatment suppressed early embryo development by inhibiting blastocyst cell numbers, and increasing blastocyst apoptosis. Our findings suggested that BDNF signaling plays important paracrine roles during blastocyst development by promoting the development of preimplantation embryos.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
香蕉觅云应助研友_nv2krn采纳,获得20
刚刚
刚刚
1秒前
大力沛萍完成签到,获得积分10
1秒前
qqqq发布了新的文献求助10
2秒前
YUE发布了新的文献求助10
2秒前
罗杰完成签到,获得积分10
3秒前
夏硕完成签到,获得积分10
3秒前
EthanYeung完成签到,获得积分10
4秒前
5秒前
yyllcc发布了新的文献求助10
5秒前
Holdon关注了科研通微信公众号
5秒前
6秒前
无花果应助EKKOO采纳,获得10
6秒前
8秒前
坚强依波发布了新的文献求助30
8秒前
YUE完成签到,获得积分10
9秒前
9秒前
10秒前
11秒前
zc完成签到,获得积分20
11秒前
13秒前
大模型应助uh采纳,获得10
13秒前
含糊的白开水完成签到,获得积分10
13秒前
14秒前
沉静盼易发布了新的文献求助10
14秒前
李爱国应助xx采纳,获得10
15秒前
个性问寒完成签到,获得积分10
15秒前
16秒前
17秒前
斯文败类应助闻元杰采纳,获得10
18秒前
18秒前
18秒前
燚一完成签到,获得积分10
19秒前
77发布了新的文献求助10
19秒前
21秒前
saisyo发布了新的文献求助10
21秒前
坚强依波发布了新的文献求助10
22秒前
22秒前
22秒前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Effect of reactor temperature on FCC yield 2000
Very-high-order BVD Schemes Using β-variable THINC Method 1020
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
Near Infrared Spectra of Origin-defined and Real-world Textiles (NIR-SORT): A spectroscopic and materials characterization dataset for known provenance and post-consumer fabrics 610
Mission to Mao: Us Intelligence and the Chinese Communists in World War II 600
Promoting women's entrepreneurship in developing countries: the case of the world's largest women-owned community-based enterprise 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3304724
求助须知:如何正确求助?哪些是违规求助? 2938716
关于积分的说明 8489688
捐赠科研通 2613208
什么是DOI,文献DOI怎么找? 1427182
科研通“疑难数据库(出版商)”最低求助积分说明 662907
邀请新用户注册赠送积分活动 647547