Effect of the probiotic preparation PDFM on sheat fish yield and intestinal microflora content has been investigated in this paper. The experimental fish group was fed pelleted diet containing 40.06% of proteins with additional 50 g of probiotic per 100 kg of diet composed of 2 x 10 (8)/ g of Enterococcus faecium bacteria. After 58 feeding days the experimental fish group achieved higher gain by 10.85% (P<0.01) compared to the control one. Results of bacteriological analyses of intestinal microflora of sheat fish fry indicate that the lactic-acidic bacterium E. faecium affected reduction of harmful bacteria Staphylococcus aureus, Escherichia coli, as well as bacterium from the family Enterobacteriaceae and complete elimination of Clostridium spp. after two week feeding.