已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Construction of novel coumarin-carbazole-based fluorescent probe for tracking of endogenous and exogenous H2S in vivo with yellow-emission and large Stokes shift

荧光 斯托克斯位移 化学 体内 内生 生物物理学 生物化学 量子力学 生物 物理 生物技术
作者
Xu Tong,Liguo Hao,Xue Song,Shuang Wu,Na Zhang,Zhongtao Li,Song Chen,Peng Hou
出处
期刊:Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [Elsevier BV]
卷期号:279: 121445-121445 被引量:9
标识
DOI:10.1016/j.saa.2022.121445
摘要

Recent medical studies have confirmed that endogenous H2S serves as the third gas-messenger besides nitric oxide (NO) and carbon monoxide (CO), which is produced by enzyme-catalyzed metabolism of cysteine and takes part in multiple physiological processes. The abnormal levels induced by H2S overproduction in mammals can destroy tissues and organ systems, which lead to certain serious diseases, such as neurodegenerative diseases, cardiovascular diseases, and various cancers. In this work, we developed a novel coumarin-carbazole fluorescent probe COZ-DNB with yellow emission and a large Stokes shift for H2S detection. In probe COZ-DNB, the newly dye COZ-OH as a luminophore and the 2,4-dinitrophenyl ether moiety was chosen as a trigger group for H2S. Probe COZ-DNB itself displayed nearly non-fluorescent. However, COZ-DNB gave the remarkable fluorescence with an 83-fold enhancement in the yellow region after interaction with H2S. The sensing mechanism of COZ-DNB toward H2S was checked by means of UHPLC, HRMS and DFT/TD-DFT calculations. What's more, probe COZ-DNB also exhibited fast response (2.0 min), high sensitivity (65.0 nM), a large Stokes shift (161.0 nm), high stability and excellent selectivity. Furthermore, COZ-DNB was applied for imaging of exogenous and endogenous H2S in living HeLa cells and zebrafish with satisfactory performances. We anticipate COZ-DNB would be served as a potential tool for investigating the biological functions of H2S in pathological processes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
飞快的孱完成签到,获得积分10
3秒前
Kevin完成签到,获得积分10
5秒前
Steven发布了新的文献求助10
6秒前
8秒前
Andy完成签到,获得积分10
8秒前
科研女仆完成签到 ,获得积分10
9秒前
沐风发布了新的文献求助10
11秒前
年轻幻柏完成签到,获得积分10
11秒前
淡定从霜完成签到 ,获得积分10
12秒前
小凯完成签到 ,获得积分10
13秒前
orixero应助芯之痕采纳,获得10
17秒前
搜集达人应助田一点采纳,获得10
20秒前
21秒前
22秒前
苗条的立果完成签到 ,获得积分10
22秒前
26秒前
27秒前
2025alex完成签到,获得积分10
28秒前
Ava应助zwd采纳,获得10
30秒前
hh完成签到 ,获得积分10
30秒前
34秒前
沐风发布了新的文献求助10
36秒前
38秒前
HRZ完成签到 ,获得积分10
41秒前
Xhh发布了新的文献求助10
42秒前
柚子完成签到 ,获得积分10
43秒前
李爱国应助整齐谷芹采纳,获得30
43秒前
Doki发布了新的文献求助10
48秒前
YDX驳回了孙孙应助
51秒前
小汤完成签到 ,获得积分20
53秒前
路冰完成签到,获得积分10
53秒前
56秒前
Ava应助典雅的俊驰采纳,获得10
59秒前
爱河完成签到,获得积分10
59秒前
温暖元容发布了新的文献求助10
59秒前
叶雨思空完成签到 ,获得积分10
1分钟前
李健应助哈哈哈哈采纳,获得10
1分钟前
zwd发布了新的文献求助10
1分钟前
Xhh完成签到,获得积分10
1分钟前
1111chen完成签到 ,获得积分10
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976583
求助须知:如何正确求助?哪些是违规求助? 3520659
关于积分的说明 11204399
捐赠科研通 3257298
什么是DOI,文献DOI怎么找? 1798683
邀请新用户注册赠送积分活动 877842
科研通“疑难数据库(出版商)”最低求助积分说明 806595