脚手架
材料科学
生物医学工程
组织工程
细胞外基质
软骨细胞
软骨
骨整合
体内
聚己内酯
植入
聚合物
解剖
复合材料
化学
外科
医学
生物技术
生物
生物化学
作者
Joseph A. M. Steele,Axel C. Moore,Jean‐Philippe St‐Pierre,Seth D. McCullen,Adam J. Gormley,Conor C. Horgan,Cameron Black,Christoph Meinert,Travis J. Klein,Siamak Saifzadeh,Roland Steck,Jiongyu Ren,Maria A. Woodruff,Molly M. Stevens
出处
期刊:Biomaterials
[Elsevier]
日期:2022-07-01
卷期号:286: 121548-121548
被引量:29
标识
DOI:10.1016/j.biomaterials.2022.121548
摘要
Articular cartilage is comprised of zones that vary in architecture, extracellular matrix composition, and mechanical properties. Here, we designed and engineered a porous zonal microstructured scaffold from a single biocompatible polymer (poly [ϵ-caprolactone]) using multiple fabrication strategies: electrospinning, spherical porogen leaching, directional freezing, and melt electrowriting. With this approach we mimicked the zonal structure of articular cartilage and produced a stiffness gradient through the scaffold which aligns with the mechanics of the native tissue. Chondrocyte-seeded scaffolds accumulated extracellular matrix including glycosaminoglycans and collagen II over four weeks in vitro. This prompted us to further study the repair efficacy in a skeletally mature porcine model. Two osteochondral lesions were produced in the trochlear groove of 12 animals and repaired using four treatment conditions: (1) microstructured scaffold, (2) chondrocyte seeded microstructured scaffold, (3) MaioRegen™, and (4) empty defect. After 6 months the defect sites were harvested and analyzed using histology, micro computed tomography, and Raman microspectroscopy mapping. Overall, the scaffolds were retained in the defect space, repair quality was repeatable, and there was clear evidence of osteointegration. The repair quality of the microstructured scaffolds was not superior to the control based on histological scoring; however, the lower score was biased by the lack of histological staining due to the limited degradation of the implant at 6 months. Longer follow up studies (e.g., 1 yr) will be required to fully evaluate the efficacy of the microstructured scaffold. In conclusion, we found consistent scaffold retention, osteointegration, and prolonged degradation of the microstructured scaffold, which we propose may have beneficial effects for the long-term repair of osteochondral defects.
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