The Effects of Plant Growth Substances on In vitro Fiber Development from Fertilized Cotton Ovules

American Journal of BotanyVolume 60, Issue 2 p. 130-139 Article THE EFFECTS OF PLANT GROWTH SUBSTANCES ON IN VITRO FIBER DEVELOPMENT FROM FERTILIZED COTTON OVULES C. A. Beasley, C. A. Beasley Department of Biology, University of California, RiversideSearch for more papers by this authorIrwin P. Ting, Irwin P. Ting Department of Biology, University of California, RiversideSearch for more papers by this author C. A. Beasley, C. A. Beasley Department of Biology, University of California, RiversideSearch for more papers by this authorIrwin P. Ting, Irwin P. Ting Department of Biology, University of California, RiversideSearch for more papers by this author First published: 01 February 1973 https://doi.org/10.1002/j.1537-2197.1973.tb10209.xCitations: 104 We gratefully acknowledge the financial support of Cotton Incorporated (3901 Barret Dr., Raleigh, North Carolina); the assistance of Ray Palmer and Phil Reid for conducting the analysis for ethylene; the excellent technical assistance of Polly Hilsabeck, Leann Feigan, and Patty Watkins; and the encouragement and support of Harold Lewis, Mack Dugger, and Lowell Lewis. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Cotton ovules were aseptically removed from ovaries 48 hr after anthesis and floated on the surface of liquid medium. Plant growth substances were filter sterilized and added to the medium in which glucose was the principal source of carbohydrate and KNO3 the sole source of nitrogen. The amount of total fiber produced from the ovule surface was determined colorimetrically from the intensity of a destaining solution to which the ovules had been transferred following their 15-second emersion in a solution of toluidine blue O. Gibberellic acid induced a marked stimulation and kinetin and abscisic acid a marked inhibition of fiber production from fertilized cotton ovules. Indoleacetic acid afforded only a trend of stimulation of fiber production. Gibberellic acid overcame the inhibition of total fiber production induced by both kinetin and abscisic acid. Indoleacetic acid did not overcome the inhibition of total fiber production induced by kinetin but did, to a great extent, overcome the inhibition due to abscisic acid. Citing Literature Volume60, Issue2February 1973Pages 130-139 RelatedInformation