纳米金刚石
表面改性
共价键
生物素化
热重分析
钻石
链霉亲和素
辣根过氧化物酶
材料科学
傅里叶变换红外光谱
化学
化学工程
核化学
生物素
有机化学
无机化学
物理化学
生物化学
工程类
酶
作者
Anke Krueger,Jochen Stegk,Yuejiang Liang,Li Lu,Gerald Jarre
出处
期刊:Langmuir
[American Chemical Society]
日期:2008-03-01
卷期号:24 (8): 4200-4204
被引量:195
摘要
We have developed a simple and efficient method for the covalent functionalization of detonation nanodiamond. After homogenization of the surface by borane reduction, the surface was modified with (3-aminopropyl)trimethoxysilane. Subsequent grafting of biotin yielded covalently biotinylated nanodiamond, which was characterized by FTIR spectroscopy, X-ray powder diffractometry, thermogravimetry, and elemental analysis. The activity was tested with horseradish peroxidase-labeled streptavidin. The surface loading of biotin was found to be 1.45 mmol g-1. The new material opens the way to covalently bonded diamond bioconjugates for labeling, drug delivery, and other applications.
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