聚乙烯亚胺
核酸
聚丙烯酸
遗传增强
转染
基因传递
分子生物学
报告基因
材料科学
癌症研究
生物
基因
生物化学
基因表达
复合材料
聚合物
作者
Sara Prijic,Lara Prosen,Maja Čemažar,Janez Ščančar,Rok Romih,Jaka Lavrenčak,Vladimir B. Bregar,Andrej Coer,Mojca Kržan,Andrej Žnidaršić,Gregor Serša
出处
期刊:Biomaterials
[Elsevier]
日期:2012-06-01
卷期号:33 (17): 4379-4391
被引量:103
标识
DOI:10.1016/j.biomaterials.2012.02.061
摘要
Cancer immuno-gene therapy is an introduction of nucleic acids encoding immunostimulatory proteins, such as cytokine interleukin 12 (IL-12), into somatic cells to stimulate an immune response against a tumor. Various methods can be used for the introduction of nucleic acids into cells; magnetofection involves binding of nucleic acids to magnetic nanoparticles with subsequent exposure to an external magnetic field. Here we show that surface modified superparamagnetic iron oxide nanoparticles (SPIONs) with a combination of polyacrylic acid (PAA) and polyethylenimine (PEI) (SPIONs-PAA-PEI) proved to be safe and effective for magnetofection of cells and tumors in mice. Magnetofection of cells with plasmid DNA encoding reporter gene using SPIONs-PAA-PEI was superior in transfection efficiency to commercially available SPIONs. Magnetofection of murine mammary adenocarcinoma with plasmid DNA encoding IL-12 using SPIONs-PAA-PEI resulted in significant antitumor effect and could be further refined for cancer immuno-gene therapy.
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