细胞生物学
磷酸化
脱磷
T细胞受体
激酶
磷酸酶
Src家族激酶
酪氨酸蛋白激酶
生物
原癌基因酪氨酸蛋白激酶Src
信号转导
蛋白质酪氨酸磷酸酶
化学
生物化学
T细胞
SH3域
免疫学
免疫系统
作者
Enfu Hui,Ronald D. Vale
摘要
Different kinases and phosphatases control the triggering of T-cell receptors. This signaling network is now reconstituted in vitro with CD3ζ, Lck, CD45, Csk and liposomes. Rigorous quantitative analyses reveal how the system is maintained in a quiescent state and how the kinase-phosphatase balance can be modulated by different events to allow activation in an ultrasensitive manner. T-cell receptor (TCR) phosphorylation is controlled by a complex network that includes Lck, a Src family kinase (SFK), the tyrosine phosphatase CD45 and the Lck-inhibitory kinase Csk. How these competing phosphorylation and dephosphorylation reactions are modulated to produce T-cell triggering is not fully understood. Here we reconstituted this signaling network using purified enzymes on liposomes, recapitulating the membrane environment in which they normally interact. We demonstrate that Lck's enzymatic activity can be regulated over an ~10-fold range by controlling its phosphorylation state. By varying kinase and phosphatase concentrations, we constructed phase diagrams that reveal ultrasensitivity in the transition from the quiescent to the phosphorylated state and demonstrate that co-clustering TCR and Lck or detaching Csk from the membrane can trigger TCR phosphorylation. Our results provide insight into the mechanism of TCR signaling as well as other signaling pathways involving SFKs.
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