丁醇
醇脱氢酶
生物化学
代谢工程
酵母
酿酒酵母
苹果酸合酶
异丁醇
生物生产
生物合成
异源的
化学
生物
酶
乙醛酸循环
异柠檬酸裂解酶
酒
基因
乙醇
作者
Anastasia Krivoruchko,Cristina Serrano-Amatriain,Yun Chen,Verena Siewers,Jens Nielsen
出处
期刊:Journal of Industrial Microbiology & Biotechnology
[Oxford University Press]
日期:2013-09-01
卷期号:40 (9): 1051-1056
被引量:107
标识
DOI:10.1007/s10295-013-1296-0
摘要
Abstract Recently, butanols (1-butanol, 2-butanol and iso-butanol) have generated attention as alternative gasoline additives. Butanols have several properties favorable in comparison to ethanol, and strong interest therefore exists in the reconstruction of the 1-butanol pathway in commonly used industrial microorganisms. In the present study, the biosynthetic pathway for 1-butanol production was reconstructed in the yeast Saccharomyces cerevisiae. In addition to introducing heterologous enzymes for butanol production, we engineered yeast to have increased flux toward cytosolic acetyl-CoA, the precursor metabolite for 1-butanol biosynthesis. This was done through introduction of a plasmid-containing genes for alcohol dehydrogenase (ADH2), acetaldehyde dehydrogenase (ALD6), acetyl-CoA synthetase (ACS), and acetyl-CoA acetyltransferase (ERG10), as well as the use of strains containing deletions in the malate synthase (MLS1) or citrate synthase (CIT2) genes. Our results show a trend to increased butanol production in strains engineered for increased cytosolic acetyl-CoA levels, with the best-producing strains having maximal butanol titers of 16.3 mg/l. This represents a 6.5-fold improvement in butanol titers compared to previous values reported for yeast and demonstrates the importance of an improved cytosolic acetyl-CoA supply for heterologous butanol production by this organism.
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