High-Content Screening for Assessing Nanomaterial Toxicity

纳米材料 活力测定 细胞内 活性氧 毒性 高含量筛选 材料科学 体外 细胞生物学 细胞 生物物理学 纳米技术 化学 生物 生物化学 有机化学
作者
Lingling Huo,Rui Chen,Xiaofei Shi,Ru Bai,Peng Wang,Yanzhong Chang,Chunying Chen
出处
期刊:Journal of Nanoscience and Nanotechnology [American Scientific Publishers]
卷期号:15 (2): 1143-1149 被引量:18
标识
DOI:10.1166/jnn.2015.9032
摘要

With rapid development of novel nanomaterials (NMs), the state of the art technologies with high efficiency and high-throughput characteristics had been applied for nanosafety evaluation. High-content screening (HCS), a cell-based multi-parametric image analysis technique, was adopted in the evaluation of eight different NMs in this study. A set of different endpoints including reactive oxygen species (ROS) production, Ca2+ transient, mitochondrial membrane potential (MMP) and cellular pH levels were checked in human bronchial epithelial (16HBE) cells after incubating with NMs for 24 hours. All NMs induced significant increase of intracellular ROS levels in 16HBE cells, although the decrease of cell viability was only found in Ag and ZnO NMs-treated cells. MMP level had a dose-response decrease in Ag, ZnO and CeO2 NMs-treated cells, while showed a significant increase in TiO2 NMs-treated cells. All tested NMs showed significant up-regulation of cellular lysosomal pH levels. However, none of NMs caused significant changes in cellular Ca2+ level at 24-hour time point. HCS allows for efficient and reliable screening of multiple responses of cells simultaneously within one screen test, which can avoid the problematic interpretation of investigations when carried on a single toxicological endpoint. Therefore, the present data provide insight and inspiration that HCS is an effective and powerful method for image-based assessments with a broad set of biological endpoints in toxicity evaluation of nanomaterials.
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