High-Content Screening for Assessing Nanomaterial Toxicity

纳米材料 活力测定 细胞内 活性氧 毒性 高含量筛选 材料科学 体外 细胞生物学 细胞 生物物理学 纳米技术 化学 生物 生物化学 有机化学
作者
Lingling Huo,Rui Chen,Xiaofei Shi,Ru Bai,Peng Wang,Yanzhong Chang,Chunying Chen
出处
期刊:Journal of Nanoscience and Nanotechnology [American Scientific Publishers]
卷期号:15 (2): 1143-1149 被引量:18
标识
DOI:10.1166/jnn.2015.9032
摘要

With rapid development of novel nanomaterials (NMs), the state of the art technologies with high efficiency and high-throughput characteristics had been applied for nanosafety evaluation. High-content screening (HCS), a cell-based multi-parametric image analysis technique, was adopted in the evaluation of eight different NMs in this study. A set of different endpoints including reactive oxygen species (ROS) production, Ca2+ transient, mitochondrial membrane potential (MMP) and cellular pH levels were checked in human bronchial epithelial (16HBE) cells after incubating with NMs for 24 hours. All NMs induced significant increase of intracellular ROS levels in 16HBE cells, although the decrease of cell viability was only found in Ag and ZnO NMs-treated cells. MMP level had a dose-response decrease in Ag, ZnO and CeO2 NMs-treated cells, while showed a significant increase in TiO2 NMs-treated cells. All tested NMs showed significant up-regulation of cellular lysosomal pH levels. However, none of NMs caused significant changes in cellular Ca2+ level at 24-hour time point. HCS allows for efficient and reliable screening of multiple responses of cells simultaneously within one screen test, which can avoid the problematic interpretation of investigations when carried on a single toxicological endpoint. Therefore, the present data provide insight and inspiration that HCS is an effective and powerful method for image-based assessments with a broad set of biological endpoints in toxicity evaluation of nanomaterials.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
WD完成签到 ,获得积分10
1秒前
2秒前
酷波er应助三和小神采纳,获得10
3秒前
cailiaokexue完成签到,获得积分10
4秒前
大个应助雨落采纳,获得10
4秒前
zz完成签到,获得积分10
6秒前
whq531608发布了新的文献求助30
6秒前
像心跳完成签到 ,获得积分10
7秒前
9秒前
9秒前
11秒前
12秒前
雨落完成签到,获得积分10
12秒前
enli完成签到,获得积分10
13秒前
寒冷晓凡发布了新的文献求助10
14秒前
Akim应助迷路以筠采纳,获得10
16秒前
23秒前
珊珊完成签到 ,获得积分10
26秒前
Shuai发布了新的文献求助10
27秒前
迷路以筠发布了新的文献求助10
28秒前
寒冷晓凡完成签到,获得积分10
30秒前
chenhunhun完成签到,获得积分10
31秒前
tingting完成签到,获得积分10
34秒前
隐形曼青应助冷言采纳,获得10
35秒前
35秒前
任性的梦菲完成签到,获得积分10
36秒前
香蕉觅云应助Kamelia采纳,获得10
36秒前
最好的完成签到,获得积分10
36秒前
36秒前
YAAAO发布了新的文献求助10
37秒前
落竹完成签到,获得积分10
38秒前
css1997完成签到 ,获得积分10
39秒前
zzl发布了新的文献求助10
39秒前
lin关闭了lin文献求助
41秒前
检检边lin完成签到,获得积分10
42秒前
科研小白完成签到 ,获得积分10
42秒前
科目三应助浮名半生采纳,获得10
46秒前
zzl完成签到,获得积分10
47秒前
CipherSage应助科研通管家采纳,获得10
47秒前
48秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 820
England and the Discovery of America, 1481-1620 600
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3572296
求助须知:如何正确求助?哪些是违规求助? 3142501
关于积分的说明 9448015
捐赠科研通 2843973
什么是DOI,文献DOI怎么找? 1563103
邀请新用户注册赠送积分活动 731630
科研通“疑难数据库(出版商)”最低求助积分说明 718640