Assessing Wound Healing in Vivo Using a Dual-Function Phosphorescent Probe Sensitive to Tissue Oxygenation and Regenerating Collagen

磷光 伤口愈合 材料科学 生物医学工程 再生(生物学) 体内 慢性伤口 生物物理学 荧光 医学 外科 光学 细胞生物学 生物 物理 生物技术
作者
Xiaoyan Wang,Zhiming Zhang,Xuhao Ye,Liping Chen,Weiming Zheng,Ning Zeng,Zhouji Shen,Fei Guo,Igor O. Koshevoy,Kristina S. Kisel,Pi‐Tai Chou,Tzu‐Ming Liu
出处
期刊:ACS Applied Materials & Interfaces [American Chemical Society]
标识
DOI:10.1021/acsami.4c15069
摘要

Levels of tissue oxygenation and collagen regeneration are critical indicators in the early evaluation of wound healing. Traditionally, these factors have been assessed using separate instruments and different methodologies. Here, we adopt the spatially averaged phosphorescence lifetime approach using ReI-diimine complexes (ReI-probe) to enable simultaneous quantification of these two critical factors in healing wounds. The topically applied, biocompatible ReI-probe penetrates wound tissue effectively and selectively binds to collagen fibers. During collagen regeneration, the phosphorescence lifetimes of the collagen-bound probe significantly extend from an initial range of 4.5–6.5 μs on day 0 to 5.5–8.5 μs by day 7. Concurrently, unbound probes in the tissue interstitial spaces exhibit a phosphorescence lifetime of 4.5–5.2 μs, revealing the oxygenation states. Using phosphorescence lifetime imaging microscopy (PLIM) and a frequency domain phosphorescence lifetime measurement (FD-PLM) system, we validated the dual-functionality of this ReI-probe in differentiating healing stages in chronic wounds. With its noninvasive, quantitative measurement capabilities for cutaneous wounds, this ReI-probe-based approach offers promising potential for early wound healing diagnosis.
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