Rapid Colorimetric Detection of Sulfite in Red Wine Using Alginate-Copper Laccase Nanozyme with Smartphone as an Optical Readout

Compared with the conventional analytical methods, nanozyme-based colorimetric sensors offer simpler and more accessible solutions for point-of-need food safety monitoring. Herein, Alginate-Cu (AlgCu) is reported as a robust laccase mimetic nanozyme for the colorimetric detection of sulfite in red wine, a common preservative in winemaking. AlgCu represents a rational design of nanozymes where the multifunctional group alginate is used as a coordination environment for the Cu catalytic center, mimicking the amino acids microenvironment in the natural laccase. The laccase activity of the AlgCu is evaluated using 2,4-dichlorophenol as a model substrate, where its oxidized product reacts with 4-aminoantipyrine, forming a reddish-pink compound with an absorption peak at 510 nm. The result showed that the AlgCu exhibited 32.81% higher laccase activity than pristine copper NPs, highlighting the role of a coordination environment in improving catalytic activity. The addition of sulfite decreased the intensity of the catalytic chromogenic product, confirming that sulfite inhibited the laccase mimetic activity of AlgCu. The observed inhibition is linearly related to the sulfite concentration from 2 to 100 μM (R ² = 0.996), enabling the detection of sulfite down to 0.78 μM. Furthermore, a sulfite concentration down to 4.9 μM could be detected by integrating the colorimetric assay with smartphone color readouts. Analysis of sulfite-spiked red wine samples gave recoveries between 96 and 106%. Overall, the obtained analytical figures of merits signify AlgCu as a robust nanozyme-based colorimetric chemosensor suitable for a point-of-need application in wine quality control and food safety monitoring in general.