继代培养(生物学)
成纤维细胞
肌成纤维细胞
体外
肾
分离(微生物学)
病理
生物
细胞生物学
医学
内科学
生物信息学
微生物学
遗传学
纤维化
作者
Tim D. Hewitson,Edward R. Smith
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 13-29
标识
DOI:10.1007/978-1-0716-3179-9_2
摘要
The renal fibroblast, and phenotypically related myofibroblast, are universally present in all forms of progressive kidney disease. The in vitro study of the fibroblast, its behaviour, and factors affecting its activity is therefore key to understanding both its role and significance. In this protocol, we describe a reproducible method for selective propagation and culture of primary renal fibroblasts from kidney cortex. Techniques for their isolation, subculture, characterization, and cryogenic storage and retrieval are described in detail.
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