半纤维素
萃取(化学)
细胞壁
制浆造纸工业
化学
废物管理
生化工程
纤维素
色谱法
工程类
有机化学
生物化学
作者
Cristine Soares Vidal,Alexandre Sousa,Lorena Silva,Tigressa H.S. Rodrigues,Edy Sousa de Brito,Nágila Maria Pontes Silva Ricardo
标识
DOI:10.1016/j.carbpol.2024.122769
摘要
The efficient repurposing of agro-industrial waste has significantly enhanced the utilization of food resources. This study aims to propose a methodology for extracting cell wall polysaccharides from residues of mango (Mangifera indica), passion fruit (Passiflora edulis), and cashew (Anacardium occidentale). Polysaccharide fractions were obtained through sequential extraction protocols involving water, cyclohexane-trans-1,2-diamine tetracetate (CDTA), sodium carbonate:CDTA, and potassium hydroxide. These fractions were categorized as water-soluble pectin (WSP), CDTA-soluble pectin (CSP), sodium carbonate-soluble pectin (SSP), and hemicellulose (HC), respectively. Each polysaccharide fraction was characterized by Nuclear Magnetic Resonance (NMR) spectroscopy and Gel Permeation Chromatography (GPC). Monosaccharide composition was determined using Gas Chromatography-Mass Spectrometry (GC-MS). NMR spectra of WSP, CSP, and SSP fractions exhibited characteristic pectin features, while the HC fraction primarily comprised hemicellulose. Consequently, the proposed methodology demonstrates potential as a standardized protocol for the extraction of pectin and hemicellulose from various food sources.
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