肝细胞癌
医学
预测标记
病理
癌症研究
信使核糖核酸
内科学
肿瘤科
癌症
基因
生物
生物化学
作者
Zhaodan Xin,Hao Chen,Jingtong Xu,Haili Zhang,Yufu Peng,Jing Ren,Qian Guo,Jiajia Song,Lin Jiao,Lei You,Linquan Bai,Yonggang Wei,Juan Zhou,Binwu Ying
摘要
Abstract Background and Aim There is a pressing need for non‐invasive preoperative prediction of microvascular invasion (MVI) in hepatocellular carcinoma (HCC). This study investigates the potential of exosome‐derived mRNA in plasma as a biomarker for diagnosing MVI. Methods Patients with suspected HCC undergoing hepatectomy were prospectively recruited for preoperative peripheral blood collection. Exosomal RNA profiling was conducted using RNA sequencing in the discovery cohort, followed by differential expression analysis to identify candidate targets. We employed multiplexed droplet digital PCR technology to efficiently validate them in a larger sample size cohort. Results A total of 131 HCC patients were ultimately enrolled, with 37 in the discovery cohort and 94 in the validation cohort. In the validation cohort, the expression levels of RSAD2, PRPSAP1, and HOXA2 were slightly elevated while CHMP4A showed a slight decrease in patients with MVI compared with those without MVI. These trends were consistent with the findings in the discovery cohort, although they did not reach statistical significance ( P > 0.05). Notably, the expression level of exosomal PRPSAP1 in plasma was significantly higher in patients with more than 5 MVI than in those without MVI (0.147 vs 0.070, P = 0.035). Conclusion This study unveils the potential of exosome‐derived PRPSAP1 in plasma as a promising indicator for predicting MVI status preoperatively.
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